Journal: Cells

Article Title: Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway

doi: 10.3390/cells11101690

Figure Lengend Snippet: Effects of platinum chemotherapeutic drugs on the levels of gonadotropin, melatonin, and oxidative stress in the peripheral blood of male gastrointestinal tumor patients, and reducing the expression of MT1/MT2 in the testis. ( A ) Human testicular tissue was stained with H&E (scale bar = 50 μm). ( B ) Images of TUNEL staining in human testicular tissue (scale bar = 50 μm). ( C ) Representative immunofluorescent pictures of MT1 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT1 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( D ) Representative immunofluorescent pictures of MT2 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT2 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( E ) MT1 mRNA expression in human testicular tissue. ( F ) MT2 mRNA expression in human testicular tissue. ( G ) GnRH level in human serum. ( H ) FSH level in human serum. ( I ) LH level in human serum. ( J ) Testosterone level in human serum. ( K ) Melatonin level in human serum. ( L ) Content of MDA in human serum. ( M ) SOD activity in human serum. ( N ) Total antioxidant capacity in human serum. Data are presented as the mean ± SEM. N = 3 cases per group in ( A – F ). N = 30 cases per group in ( G – N ). CT, chemotherapy. * p < 0.05, ** p < 0.01 versus the control group or the tumor group.

Article Snippet: A Mouse MT (melatonin) ELISA Kit (Cat#: E-EL-M0788c), Human MT (melatonin) ELISA Kit (Cat#: E-EL-H2016c), GnRH (gonadotropin-releasing hormone) ELISA Kit (Cat#: E-EL-0071c), T (testosterone) ELISA Kit (Cat#: E-EL-0155c), Human FSH (follicle stimulating hormone) ELISA Kit (Cat#: E-EL-H1143c), and Human LH (luteinizing hormone) ELISA Kit (Cat#: E-EL-H6019) were purchased from Elabscience (Wuhan, China).

Techniques: Expressing, Staining, TUNEL Assay, Fluorescence, Labeling, Activity Assay, Control

Journal: Frontiers in Molecular Neuroscience

Article Title: Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function

doi: 10.3389/fnmol.2024.1459858

Figure Lengend Snippet: FSH induces neuroinflammation in mice. Four weeks after the FSH treatment, the brain and serum of the mice were harvested for western blot (a,b), Elisa assay (c), and immunofluorescence staining (d,e). (a) Representative immunoblots showing the protein expression of IL-1β and IL-6 in the hippocampus. (b) Quantification of the immunoreactivity of IL-1β and IL-6 protein expression, data are shown as represent mean ± SEM, n = 4 mice per group. (c) ELISA quantification shows the levels of proinflammation factors IL-1β, IL-6, and TNFα in the serum, data are shown as mean ± SEM, n = 6 mice per group. (d) Immunofluorescent co-staining of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm). (e) Quantification of Iba1 positive and GFAP positive cells, data represent mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, * *p < 0.01; ns, not significant.

Article Snippet: Recombinant human FSH protein (hor-249) was from Prospecbio; antibody to IL-6 (YT5348) was from Immuneway; antibodies to IL-1 β (16806-1-AP), GluR1 (67642-1-Ig), FSHR (22665-1-AP), GAD67 (10408-1-AP), VGluT1 (21829-1-AP), and synaptophysin (17785-1-AP) were from Proteintech; antibodies to GluR2 (A11316), synapsin (WX620330), PSD95 (A7889), ERK1/2 (A4782), and phospho-ERK1/2 (AP0472) were from Abclonal; antibody to GFAP (AB-2532994) was from Invitrogen; antibody to Iba1 (019-19741) was from Wako; antibody to β-actin (GB11001-100) was from Servicebio; ERK1/2 inhibitor (HY-112287) was from MedChemExpress; IL-1β mouse ELISA kit (MU30369), IL-6 mouse ELISA kit (MU30044), and TNF- α mouse ELISA kit (MU30030) were from Bioswamp; human FSH ELISA kit (CSB-E06867h) and luteinizing hormone (LH) ELISA kit (CSB-E12770m) were from Cusabio; 17β-estradiol ELISA kit (ab108667) was from Abcam; 4′,6-diamidino-2-phenylindole (DAPI, D9542) was from Sigma-Aldrich; AAV9 sh-FSHR (BC-2403) and AAV9 sh-Control (BC-0183) adenovirus were customized, synthesized, and purified by Shenzhen Brain Case Biotechnology Inc.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing

Journal: Frontiers in Molecular Neuroscience

Article Title: Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function

doi: 10.3389/fnmol.2024.1459858

Figure Lengend Snippet: FSHR knockdown alleviates FSH-induced neuroinflammation in mice. (a,b) Representative immunoblots images (a) and quantification (b) of IL-1β and IL-6 protein expression showing a reduction in the hippocampus of the sh-FSHR + FSH group compared to sh-Control + FSH group, data are presented as mean ± SEM, n = 4 mice per group. (c) ELISA quantification of pro-inflammation factors IL-1β, IL-6, and TNFα in the serum, data are presented as mean ± SEM, n = 6 mice per group. (d,e) Immunofluorescent co-staining (d) and quantification (e) of Iba1 (gray) and GFAP (red) on the hippocampal sections (scale bar: 150 μm), data are presented as mean ± SEM ( n = 4 mice per group, 10 sections each mouse). Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05, * *p < 0.01.

Article Snippet: Recombinant human FSH protein (hor-249) was from Prospecbio; antibody to IL-6 (YT5348) was from Immuneway; antibodies to IL-1 β (16806-1-AP), GluR1 (67642-1-Ig), FSHR (22665-1-AP), GAD67 (10408-1-AP), VGluT1 (21829-1-AP), and synaptophysin (17785-1-AP) were from Proteintech; antibodies to GluR2 (A11316), synapsin (WX620330), PSD95 (A7889), ERK1/2 (A4782), and phospho-ERK1/2 (AP0472) were from Abclonal; antibody to GFAP (AB-2532994) was from Invitrogen; antibody to Iba1 (019-19741) was from Wako; antibody to β-actin (GB11001-100) was from Servicebio; ERK1/2 inhibitor (HY-112287) was from MedChemExpress; IL-1β mouse ELISA kit (MU30369), IL-6 mouse ELISA kit (MU30044), and TNF- α mouse ELISA kit (MU30030) were from Bioswamp; human FSH ELISA kit (CSB-E06867h) and luteinizing hormone (LH) ELISA kit (CSB-E12770m) were from Cusabio; 17β-estradiol ELISA kit (ab108667) was from Abcam; 4′,6-diamidino-2-phenylindole (DAPI, D9542) was from Sigma-Aldrich; AAV9 sh-FSHR (BC-2403) and AAV9 sh-Control (BC-0183) adenovirus were customized, synthesized, and purified by Shenzhen Brain Case Biotechnology Inc.

Techniques: Knockdown, Western Blot, Expressing, Control, Enzyme-linked Immunosorbent Assay, Staining

Journal: Saudi Journal of Biological Sciences

Article Title: Pharmacological intervention of biosynthesized Nigella sativa silver nanoparticles against hexavalent chromium induced toxicity in male albino mice

doi: 10.1016/j.sjbs.2023.103570

Figure Lengend Snippet: Layout for kits used in the current study.

Article Snippet: 1 , Follicle stimulating hormone (FSH) , Immunodiagnostic reagents and Elisa Kit test (DiaMetra kits, Via Giustozzi, Italy , ( ) .

Techniques: Enzyme-linked Immunosorbent Assay, Diagnostic Assay